The Development and Implementation of a Multimedia Program that Uses Analogies in Senior High School Chemistry to Enhance Student Learning of Chemical Equilibrium

In this thesis, you will find a review the development process of a multimedia presentation designed to assist the teaching of chemical equilibrium using analogies. The objective of this thesis is to report on the process of designing animated analogies and the subsequent employment of these analogies in a teaching program for grades 11 and 12 students. This thesis describes a case study in the effective use of technology in the classroom based on established research in the field of analogical thinking. The work begins with a review of relevant literature from the fields of constructivism, collaborative learning and multimedia in education. The design phase of the research sought to discover if analogies for chemical equilibrium could be successfully transferred to a multimedia presentation on a computer. The subsequent testing of the software endeavoured to discover the most appropriate teaching strategies and if the use of such a program could enhance the learning process for students. The work resulted in a completed CDROM with full teaching program included which you will find attached to this thesis. The results indicate that the experience was a positive one and that there is some evidence to show increased ability in the students in their attempts to understand a conceptually difficult area of chemistry theory

Measuring Entangled Qutrits and Their Use for Quantum Bit Commitment

We produce and holographically measure entangled qudits encoded in transverse spatial modes of single photons. With the novel use of a quantum state tomography method that only requires two-state superpositions, we achieve the most complete characterisation of entangled qutrits to date. Ideally, entangled qutrits provide better security than qubits in quantum bit-commitment: we model the sensitivity of this to mixture and show experimentally and theoretically that qutrits with even a small amount of decoherence cannot offer increased security over qubits.Comment: Paper updated to match published version; 5 pages, 4 figures, images have been included at slightly lower quality for the archiv

Measuring Controlled-NOT and two-qubit gate operation

Accurate characterisation of two-qubit gates will be critical for any realisation of quantum computation. We discuss a range of measurements aimed at characterising a two-qubit gate, specifically the CNOT gate. These measurements are architecture-independent, and range from simple truth table measurements, to single figure measures such as the fringe visibility, parity, fidelity, and entanglement witnesses, through to whole-state and whole-gate measures achieved respectively via quantum state and process tomography. In doing so, we examine critical differences between classical and quantum gate operation.Comment: 10 pages (two-column). 1 figur

Origin-of-transfer sequences facilitate mobilisation of non-conjugative antimicrobial-resistance plasmids in Staphylococcus aureus

Staphylococcus aureus is a common cause of hospital, community and livestock-associated infections and is increasingly resistant to multiple antimicrobials. A significant proportion of antimicrobial-resistance genes are plasmid-borne, but only a minority of S. aureus plasmids encode proteins required for conjugative transfer or Mob relaxase proteins required for mobilisation. The pWBG749 family of S. aureus conjugative plasmids can facilitate the horizontal transfer of diverse antimicrobial-resistance plasmids that lack Mob genes. Here we reveal that these mobilisable plasmids carry copies of the pWBG749 origin-of-transfer (oriT) sequence and that these oriT sequences facilitate mobilisation by pWBG749. Sequences resembling the pWBG749 oriT were identified on half of all sequenced S. aureus plasmids, including the most prevalent large antimicrobial-resistance/virulence-gene plasmids, pIB485, pMW2 and pUSA300HOUMR. oriT sequences formed five subfamilies with distinct inverted-repeat-2 (IR2) sequences. pWBG749-family plasmids encoding each IR2 were identified and pWBG749 mobilisation was found to be specific for plasmids carrying matching IR2 sequences. Specificity of mobilisation was conferred by a putative ribbon-helix-helix-protein gene smpO. Several plasmids carried 2–3 oriT variants and pWBG749-mediated recombination occurred between distinct oriT sites during mobilisation. These observations suggest this relaxase-in trans mechanism of mobilisation by pWBG749-family plasmids is a common mechanism of plasmid dissemination in S. aureus

Business Models for SEEV4-City Operational Pilots: From a generic SEEV4-City business model towards improved specific OP business models

This report, led by Northumbria University, provides a final analysis by project partners regarding Business Models for SEEV4-City Operational pilots. It is part of a collection of reports published by the project covering a variation of specific and cross-cutting analysis and evaluation perspectives and spans 6 operational pilots

The Molecular Epidemiology of the Highly Virulent ST93 Australian Community Staphylococcus aureus Strain

In Australia the PVL - positive ST93-IV [2B], colloquially known as ‘‘Queensland CA-MRSA’’ has become the dominant CA-MRSA clone. First described in the early 2000s, ST93-IV [2B] is associated with skin and severe invasive infections including necrotizing pneumonia. A singleton by multilocus sequence typing (MLST) eBURST analysis ST93 is distinct from other S aureus clones. To determine if the increased prevalence of ST93-IV [2B] is due to the widespread transmission of a single strain of ST93-IV [2B] the genetic relatedness of 58 S. aureus ST93 isolated throughout Australia over an extended period were studied in detail using a variety of molecular methods including pulsed-field gel electrophoresis, spa typing, MLST, microarray DNA, SCCmec typing and dru typing. Identification of the phage harbouring the lukS-PV/lukF-PV Panton Valentine leucocidin genes, detection of allelic variations in lukS-PV/lukF-PV, and quantification of LukF-PV expression was also performed. Although ST93-IV [2B] is known to have an apparent enhanced clinical virulence, the isolates harboured few known virulence determinants. All PVL-positive isolates carried the PVL-encoding phage WSa2USA and the lukS-PV/lukF-PV genes had the same R variant SNP profile. The isolates produced similar expression levels of LukF-PV. Although multiple rearrangements of the spa sequence have occurred, the core genome in ST93 is very stable.The emergence of ST93-MRSA is due to independent acquisitions of different dru-defined type IV and type V SCCmec elements in several spa-defined ST93-MSSA backgrounds. Rearrangement of the spa sequence in ST93-MRSA has subsequently occurred in some of these strains. Although multiple ST93-MRSA strains were characterised, little genetic diversity was identified for most isolates, with PVLpositive ST93-IVa [2B]-t202-dt10 predominant across Australia. Whether ST93-IVa [2B] t202-dt10 arose from one PVL-positive ST93-MSSA-t202, or by independent acquisitions of SCCmec-IVa [2B]-dt10 into multiple PVL-positive ST93-MSSA-t202 strains is not known

SEEV4-City Policy Recommendations and Roadmap: Recommendations towards integration of transport, urban planning and energy

This report, led by Northumbria University and POLIS, provides a final analysis by project partners regarding policy recommendations and a roadmap based on the culmination of experiences, learnings and additional research within the Interreg NSR SEEV4-City project. It is part of a collection of reports published by the project covering a variation of specific and cross-cutting analysis and evaluation perspectives and spans 6 operational pilots. This report is dedicated to policies relating to the integration of transport, urban planning and energy

Seminal plasma and prostaglandin E2 up-regulate fibroblast growth factor 2 expression in endometrial adenocarcinoma cells via E-series prostanoid-2 receptor-mediated transactivation of the epidermal growth factor receptor and extracellular signal-regulated kinase pathway

We report a multiwavelength (X-ray, ultraviolet/optical/infrared, radio) analysis of the relativistic tidal disruption event candidate Sw J2058+05 from 3 months to 3 yr post-discovery in order to study its properties and compare its behavior with that of Sw J1644+57. Our main results are as follows. (1) The long-term X-ray light curve of Sw J2058+05 shows a remarkably similar trend to that of Sw J1644+57. After a prolonged power-law decay, the X-ray flux drops off rapidly by a factor of $\gtrsim 160$ within a span of $\Delta$$t$/$t$ $\le$ 0.95. Associating this sudden decline with the transition from super-Eddington to sub-Eddington accretion, we estimate the black hole mass to be in the range of $10^{4-6}$ M$_{\odot}$. (2) We detect rapid ($\lesssim 500$ s) X-ray variability before the dropoff, suggesting that, even at late times, the X-rays originate from close to the black hole (ruling out a forward-shock origin). (3) We confirm using HST and VLBA astrometry that the location of the source coincides with the galaxy's center to within $\lesssim 400$ pc (in projection). (4) We modeled Sw J2058+05's ultraviolet/optical/infrared spectral energy distribution with a single-temperature blackbody and find that while the radius remains more or less constant at a value of $63.4 \pm 4.5$ AU ($\sim 10^{15}$ cm) at all times during the outburst, the blackbody temperature drops significantly from $\sim$ 30,000 K at early times to a value of $\sim$ 15,000 K at late times (before the X-ray dropoff). Our results strengthen Sw J2058+05's interpretation as a tidal disruption event similar to Sw J1644+57.Comment: Replaced with the published version of the manuscrip